Search results for "methods comparison"

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Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…

2019

Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…

Concordancedigital droplet PCR; methods comparison; real-time PCR; torquetenovirusViremiaTTVBiologyReal-Time Polymerase Chain ReactionComparative evaluation03 medical and health sciencesTTV; digital droplet PCR; methods comparison; real-time PCR0302 clinical medicineImmune systemVirologymedicineHumans030212 general & internal medicineViremiaDigital droplet pcrTorque teno virusSurrogate endpointReproducibility of Resultsmedicine.diseaseVirologyDNA Virus InfectionsQuantitative measuretorquetenovirusInfectious DiseasesReal-time polymerase chain reactionCase-Control StudiesDNA Viral030211 gastroenterology & hepatologyreal-time PCRImmunocompetencedigital droplet PCRBiomarkersmethods comparison
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